Genotyping

 

Genotyping is the process of identifying the species and subtype of a given organism.

There are many ways of genotyping human cells or pathogens.

 

 

Methods of Genotyping

Line probe assays begin with PCR, resulting in biotin-labeled amplification products. These are incubated with a nitrocellulose strip containing various probes. Hybridization of the biotinylated products and nitrocelluose probes is followed by incubation with a streptavtidin-alkaline phosphatase reaagent, and AP is used to catalyze a chromogenic reaction.

This test can detect numerous genotypes simultaneously.