Western Blotting
last authored: March 2010, David LaPierre
last reviewed:
Introduction
Western blots used to detect antibodies against pathogens depend on prepared pathogenic proteins that are run through the gel. A patient's serum is used as a primary antibody, with anti-human secondary antibodies used as detection.
This identifies a pattern of serum antibodies, as compared with only one antibody being detected in an EIA/OIA. As such Western blotting is more specific.
Uses
Procedure
Resources and References
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